Fig. 8. Different concentration dependent effects of TSP1 on P4 production in the bubaline luteal cells cultured in vitro at 72 hour of culture. Graph also depicting the amount of progesterone in spent media of TSP1 knocked out luteal cells after 72 hour of culture. Production of P4 in the supernatant was assayed by RIA. One-way ANOVA with Tukey's HSD test was used to find the effect of concentration and time on the dependent variables like P4. Each point in the line chart indicates Mean ± SEM at 95% significance (p<0.05). Abbreviations: TSP, Thrombospondin; CL, Corpus luteum; P4, Progesterone; TSP1-KO, TSP1 knockout.